我写了一些代码，通过阅读我的文本文件，找到一组与基因相关的数字，然后用这些数字搜索基因本身，这样我就可以提取一堆包含每个基因的文本文件。我已经成功地得到了数字，但我有问题的文件写作。得到错误“local variable”gene_substring“referenced before assignation”。我做了一些研究，并尝试使用global来修复它，但它在其他地方抛出了错误。#function to extract the genes by using the numbers in my list
end_file = "/Users...."
def extract_genes(start_stop, genome_sequence):
for start,stop in start_stop:
# extracts start:stop gene from the sequence
if start > stop:
gene_substring = genome_sequence[0:start] + genome_sequence[stop:]
# store in file
with open(end_file + "/" + name + "+" + ".txt", "w",) as file:
file.write(gene_substring)#My code to get the output
work_dir = "/Users/"
for path in glob.glob(os.path.join(work_dir, "*.gbff")):
numbers = extract_numbers(path)
sequences = extract_seq(path)
extract_genes(start_stop, sequences)
print(path)
我该怎么解决这个？谢谢：)
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